FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9016973
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG146070.16199449637921728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG61900.046.1405071
TATGCCG7750.038.1126448
TCTCGTA7950.037.58655543
CTCGTAT8000.037.3520544
CGTATGC8450.035.36406746
TCGTATG8550.034.95025645
GATCGGA9450.034.561871
ACACGTC10200.032.69295513
TGCCGTC9050.031.49016450
ATCGGAA12100.028.4251542
ATGCCGT10550.028.32870549
ATCTCGT10950.028.23970842
GCCGTCT9200.027.95601351
TCGGAAG11950.027.9076043
CGTCTGA12650.026.36038816
CGGAAGA14900.022.8484484
ACGTCTG15900.022.06456415
CACGTCT15750.021.8336214
CCGTCTT12800.021.18033652
CGTCTTC12500.020.57699653
Produced by FastQC (version 0.11.5)