FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7538113
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA700.001279622724.8211843
ACGACGA1000.00861457717.86281868
CCGGAGA4902.3646862E-1112.7635735
ACACGTC6402.0008883E-1110.85696113
GATCGGA5251.2334567E-810.5907231
CGTCTGA5907.0886017E-910.01048716
ATCGGAA7201.9412255E-88.6868382
CACTCCG8101.6530976E-88.14980131
TCGGAAG7453.0939555E-77.92887263
ACTCCGG7909.3694325E-87.91638332
CGGAGAA7301.9285944E-67.61555436
GCTAACC8852.73366E-47.491089370
CGGAAGA8603.881505E-77.2727014
CTCCGGA8906.8735426E-77.02694733
CCCCGGC12554.6770096E-56.60320170
TCCGGAG9301.0170776E-56.351202534
ACGTCTG10752.3108041E-66.140495315
CACGTCT11951.3844008E-55.52387714
CACACGT13055.975254E-55.05826312
Produced by FastQC (version 0.11.5)