FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6051849
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4650.039.61466248
CGTATGC4950.035.8062246
TCTCGTA5350.033.12665643
CTCGTAT5450.032.51936744
ATGCCGT5600.031.65354549
ACCGCTC5800.031.14790732
TATCTCG5900.030.03634541
CGCTCAT5900.030.0313834
GATCGGA5850.029.1063041
GCCGTCT6150.028.82536151
TGCCGTC6200.028.5914850
CCGCTCA6200.028.57730133
GTCACCG6400.027.68403229
TCGTATG6800.026.57502245
CACCGCT7150.025.75252231
ATCGGAA7000.025.314672
ACACGTC7350.024.57645613
ATCTCGT7500.024.0930942
CCGTCTT7650.023.62849252
CGTCTTC7900.023.3215453
Produced by FastQC (version 0.11.5)