FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7055090
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4700.038.44993248
GCCGTCT5500.032.2287751
CCGATCG450.00446403930.88854645
TCTCGTA5600.029.78327243
GATCGGA6350.028.9964161
TGCCGTC6150.028.82104950
GTCACGA6500.028.32003629
AGGGGGG25850.028.0884571
CGTATGC6300.027.57984246
CTCGTAT6150.027.12049744
ATGCCGT7200.025.09961549
ATCTCGT6800.024.52670542
TCGTATG7450.024.25476545
ACGAGAT8000.023.88036332
ATCGGAA8300.023.0185242
ACACGTC8150.022.58589213
AGTCACG8500.022.06511128
CGTCTGA8550.021.52954716
CGACGCT1154.370395E-521.14413835
CCGTCTT9350.020.44617352
Produced by FastQC (version 0.11.5)