FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6922236
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87390.12624533459997608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTG3400.022.47103726
CACCGGT3750.021.29873825
GGTCGGA4150.020.903654
CGGCTTG4250.019.6281550
CAGGTCG4500.018.4991992
GTCGGAG5050.017.8671885
CCGGCTT5800.014.38321849
TCGGAGA6550.013.7768616
CGGAGAT6600.013.14814957
AGCGTCG1600.00952744913.03450856
CCACCGG6050.012.62597324
CCGGTGG6500.012.28826827
CGGTGGT7400.011.73259128
AGGTCGG7350.011.3280893
TCCCCGG7500.011.12310346
TTCCCCG7650.010.45009745
TGGACGC3500.00194403468.93775454
CAATGCC24750.08.19810870
CCCCGGC12404.0017767E-117.28837647
GGTTCCC15907.2759576E-126.557814643
Produced by FastQC (version 0.11.5)