FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7086066
Sequences flagged as poor quality0
Sequence length35-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4550.024.43009648
CTCGTAT4350.023.14144
TCTCGTA4750.021.9188743
CGGCTAT5100.021.76007834
GATCGGA4850.020.7375951
CGTATGC5200.020.70041746
TGCCGTC6000.019.11456350
TCGTATG6100.018.78074345
ATCGGAA6200.018.4576022
ATCGACC1500.007618273618.24762370
TCACCGG5750.018.09065630
GCCGTCT6000.017.9601751
ACCGGCT6400.017.33859332
CGTCTGA6850.017.20948216
ATGCCGT6450.016.69945349
TCGGAAG7700.016.2128263
ACACGTC7500.016.18028613
CACCGGC7100.016.1171131
GTCACCG6650.015.64152329
CGTCTTC7950.015.3153553
Produced by FastQC (version 0.11.5)