FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5122705
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTG800.00767151227.64808570
TATGCCG2400.024.61732748
CGTATGC3250.019.24717146
GATCGGA3300.018.953511
TGCCGTC3051.8189894E-1218.2331450
ATGCCGT3750.016.68234849
TCTCGTA3800.016.45962743
ACACGTC4050.016.29728113
TATCTCG3651.8189894E-1216.18373141
CTCGTAT4051.2732926E-1114.58633544
ATCGGAA4659.094947E-1213.4487772
GCCGTCT4254.4383341E-1013.08560151
CGTCTGA4601.1459633E-1012.83855516
AGGGGGG13850.012.795331
TCGTATG4356.4210326E-1012.7821645
CCCGCAC3300.0038520511.7294970
ATCTCGT5055.4205884E-1011.69726742
ACGTCTG6251.8189894E-1211.67251315
CGGAAGA6603.8198777E-1110.5276714
CACGTCT6553.619789E-1010.07714214
Produced by FastQC (version 0.11.5)