FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6630312
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7250.047.45760348
CGTATGC7650.044.51883746
GATCGGA8250.041.28111
CGGCTAT8400.040.9493134
TGCCGTC8850.038.88092850
CTCGTAT8450.038.24741744
TCTCGTA8700.037.54638343
GCCGTCT9000.037.4616651
ACCGGCT9200.037.3879432
TCGTATG9350.036.7961845
ACACGTC9750.035.27754613
TCACCGG9800.035.09782430
ATCGGAA10250.033.902042
ATGCCGT10400.033.41886549
ATCTCGT9800.032.6218242
TCGGAAG10750.032.0017053
GTCACCG10900.031.87434629
CACCGGC11050.031.75631731
CCGTCTT11200.030.72449952
CGTCTTC11400.030.18570153
Produced by FastQC (version 0.11.5)