FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7457961
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN201850.27065038285933646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGG6503.2014214E-1010.1441739
TAGGGGG14300.09.96538750
AGGGGGG32150.09.08098651
AGCGGGG8657.185008E-108.42552810
AGAGCGG6251.7704078E-68.3284518
GGGGGGT44750.08.14349214
GGGGGCG20350.07.51783456
GAGAGCG6855.337318E-57.0921647
GGGGGGC28500.06.70936455
GGGGGGA41800.06.6368622
GGGCGGG23200.06.596843758
CGGGGGA7501.6812916E-46.49661961
GGGGCCG8652.3668523E-56.432743557
GGGGAGA41000.06.4286924
GGGGCGG22350.06.379677357
GAGGGGG34100.06.30977259
CGGGGGG21000.06.27972612
GGGGGAG43200.06.2611083
GCGGGGG20350.06.139403311
GGGGGTG48950.05.672316615
Produced by FastQC (version 0.11.5)