FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7157465
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN119590.16708429590644175No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA95540.13348301388829703TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAA11500.062.80798735
TCCGCGA11600.062.2630534
CGCGAAA11700.061.73694236
CTCCGCG12650.056.8149133
TATGCCG13800.052.38289348
CGTATGC13850.052.1864746
TCTCGTA13900.051.7386143
GATCGGA14150.051.040361
CTCGTAT14200.050.6487344
GCGAAAT14450.050.2336537
TGCCGTC14700.049.42085650
ATGCCGT15100.048.1069749
GCCGTCT15050.048.0452851
ACTCCGC15050.047.982432
ATCTCGT15650.046.17254642
CACTCCG16050.045.85584331
ACACGTC16100.045.49271413
ATCGGAA16500.045.238342
TCGTATG16400.044.28058645
CGTCTGA17050.043.3651116
Produced by FastQC (version 0.11.5)