FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7157465
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN154560.2159423762463386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTA1900.001996056912.80509946
GGGGGCG18350.09.09377456
GGGGGGC26550.08.24862455
GGGGCGG20700.08.23049157
GAGCGGG6352.2173208E-68.1983939
GGGGGGA39000.07.8271592
CGGGGGA8402.560937E-77.456128661
AGCGGGG7502.941275E-67.404267310
GGGGGGT38950.07.307457414
AGGGGGG29200.07.26000151
GGGCGGG22000.07.113016658
AGAGCGG5905.4112973E-47.05865868
GCGGGGT6502.1491035E-46.942084314
TAGGGGG13756.366463E-116.82404150
GAGAGCG6703.120226E-46.733827
GGGGGAG40200.06.55817133
GGCGGGA9208.46697E-66.42676159
CGGGGGG19300.06.29397412
GAGGGGG32850.05.8108359
GCGGGGG19303.274181E-115.75449111
Produced by FastQC (version 0.11.5)