FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8028825
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA6300.047.40428534
CGCGAAA6300.046.8559936
CCGCGAA6500.045.9474135
TATGCCG7500.038.9224348
CTCGTAT7550.035.4322244
CGTATGC8600.034.74653246
CTCCGCG8650.034.52501733
TCTCGTA8150.032.82105643
GCGAAAT9000.032.80021737
GATCGGA9650.031.3116381
ACTCCGC10050.030.40567232
GCCGTCT10000.029.89749551
ACACGTC10800.029.57750113
TGCCGTC10000.029.54597950
TCGTATG10000.029.5325345
ATGCCGT10050.029.39531549
ATCTCGT9300.028.3872342
CGTCTTC10950.028.26209653
CGTCTGA11950.026.44057716
ATCGGAA11700.026.416792
Produced by FastQC (version 0.11.5)