FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7292827
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAACTGTCCACAGGCAACAGGGAGTCATGTCGCGGTTCTCCAGGAGCAC119910.16442183531845742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCC21600.067.43815670
TGTCGCG15300.060.40577329
GTCGCGG16150.057.2324130
TCGCGGT16600.055.89216231
ATGTCGC16550.055.63387728
CGCGGTT17150.053.9041232
GCGGTTC17800.052.32761833
CGGTTCT18150.051.32453534
CATGTCG18000.050.95960227
ACACGTC20050.050.8826369
GACACGT13900.047.33299668
AGACACG17550.037.32831667
GGAGCAC26350.035.40458344
GAGCACC26950.034.49928745
AGCACCC27900.033.83243646
ACTGTCC28850.033.477024
GTCCACA30150.032.6056337
AACTGTC30750.032.0859573
GGGAGTC31750.029.43558921
GGTTCTC31200.029.1937135
Produced by FastQC (version 0.11.5)