FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7292827
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATATCCGGGAACCTACCAGCCTATGGCCAGTTACCTGGACGTGTCTGTGGTGCAGACTCTGGGTGCTCCTGG81490.1117399329505554No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN79930.10960084477528399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGG16300.057.9327624
CGGGAAC16600.057.531858
ATATCCG16550.057.4693373
GACGTGT17650.052.81749340
TCCGGGA19350.048.6351786
ACGTGTC13450.048.44651841
CTGGACG19700.047.29945437
ATCCGGG19900.047.2867665
TGGACGT19950.046.7141238
GGACGTG21150.044.2350839
CCGGGAA21350.044.0831227
GGGAACC24500.038.9829039
CTATGGC24600.038.41193423
ACCTACC26950.036.33856613
CGTGTCT17750.036.327442
GGTGCTC26400.035.9912964
AGTTACC26550.035.98627531
GGAACCT26750.035.70617710
TATGGCC26500.035.65860724
GATATCC26900.035.3553432
Produced by FastQC (version 0.11.5)