FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7429120
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAA2300.030.20070635
TCCGCGA2550.027.23893534
CGCGAAA2600.026.7169136
TATGCCG3650.021.90065248
CTCCGCG3800.019.1920533
CGTATGC4700.017.00497446
TCTCGTA4850.016.47616243
CTCGTAT5300.013.76710544
GCGAAAT5350.013.63366537
ATGCCGT5900.013.55026149
GATCGGA5500.013.262911
GCCGTCT5800.012.58808651
GTCGATC2100.003377369611.93792368
ACACGTC6150.011.85730613
TGCCGTC6200.011.77428550
CGTCTGA6800.011.23454916
ACTCCGC7101.8189894E-1210.76057232
ATCGGAA6959.094947E-1210.4945552
TCGTATG7201.8189894E-1110.13452845
CGTCTTC7553.6379788E-1210.13354153
Produced by FastQC (version 0.11.5)