FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9506972
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG34450.030.0447481
GATCGGA7150.026.7176461
ATCGGGG6450.026.39502142
CCGGAGA8400.023.98112935
GAATCGG7450.022.38251140
AATCGGG7550.022.08768541
ATCGGAA8900.021.8507522
CGGAGAA9300.020.91434736
ACACGTC9350.020.79582413
CGTCTGA9600.020.25373516
ACTCCGG10300.019.55464632
CACTCCG10700.017.52452931
TCGGAAG11450.016.9829063
CTCCGGA12450.015.899734533
TCCGGAG12800.015.73678834
TCGGGGG8100.015.44271743
ACGTCTG14400.013.74360615
CACGTCT14950.013.7024814
CGGAAGA14650.013.2732594
GCACACG16950.011.88113811
Produced by FastQC (version 0.11.5)