FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6774309
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4850.035.10955448
GATCGGA5250.031.7662731
TCTCGTA6050.027.56577543
GCCGTCT6300.027.03137851
CTCGTAT6300.027.02399344
CGTATGC6350.026.81318546
ATGCCGT6400.026.60783249
TATCTCG6550.026.52104241
TGCCGTC6450.026.40176650
GTCACGA6500.026.18378329
TCGTATC6850.025.3572938
ATCGGAA7300.023.793292
AGTCACG7200.023.15538428
ATCTCGT7250.023.00231642
ACACGTC7250.022.99569113
CGTATCT7450.022.84980439
GAATTCG7300.022.84105334
TCGGAAG7500.022.6956253
CGTCTGA7650.022.24749616
TCGTATG7550.022.09010745
Produced by FastQC (version 0.11.5)