FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9237022
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN285200.30875751946893704No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG248060.2685497555380944No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT92700.10035701982738592TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA23150.058.4360161
TATGCCG24400.058.1398848
AGGGGGG118050.056.239691
TGCCGTC25400.056.14945250
TCTCGTA24800.056.03110543
CTCGTAT25450.054.60956644
CGTATGC26050.054.56850446
CGATAGA26100.054.11664636
ACACGTC26550.053.81202713
GCCGTCT26500.053.70032551
TCGTATG26700.053.62240245
ATGCCGT26750.053.56502549
GCGATAG26500.053.55675535
AGCGATA26500.053.54948434
TCGGAAG27350.052.367073
ATCGGAA27100.052.2137342
CGTCTGA27400.052.14069716
CAGCGAT27300.052.1022633
ATCTCGT27000.051.97178342
CGGAAGA29300.048.7624664
Produced by FastQC (version 0.11.5)