FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7265446
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3650.025.7070748
CTCGTAT4750.021.9467644
TCTCGTA4300.021.81901643
TGCCGTC4800.020.27292650
CGTATGC4900.019.85741446
ATGCCGT5550.018.78533449
CGCTCAT5600.018.61222534
ACCGCTC5200.018.03966532
GCCGTCT5250.017.87364251
GATCGGA5350.017.5362951
TCGTATG6600.016.84848245
CCGCTCA6050.016.65346733
GTCACCG6200.015.68987429
TATCTCG6100.015.37945141
ACACGTC6100.015.376698513
ATCGGAA6800.014.3065572
ATCTCGT7800.013.36464642
CACCGCT7050.013.30548631
CGTCTGA7550.012.88413316
CGTCTTC7650.012.26681653
Produced by FastQC (version 0.11.5)