FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6030304
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4300.041.21185348
CGTATGC5100.035.4265146
CTCGTAT5150.034.40618544
TCTCGTA5150.034.40561743
GCCGTCT5800.031.75680451
ATCTCGT5650.031.36008842
TACTCGA5800.030.54626736
TGCCGTC5850.030.29498750
TTACTCG5800.029.94682535
TCGTATG6450.028.01096245
GATCGGA6400.027.1420081
ATGCCGT6700.026.96935849
ATCGGAA7150.025.2631842
CGTCTTC7600.024.6941653
CGTCTGA7600.024.22118616
ACACGTC7500.024.08103813
TCGGAAG7550.023.9245383
CCGTCTT8150.023.45392852
ACTCGAT7550.023.00646237
ACGTCTG9200.020.76385515
Produced by FastQC (version 0.11.5)