FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6031945
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6850.046.6830848
CTCGTAT6300.045.77710744
TCTCGTA6450.043.0942643
CGTATGC7850.039.84466646
ACACGTC8550.038.17038713
TGCCGTC8250.037.92508750
GCCGTCT8450.037.44197551
CGTCTTC8550.036.60563353
CGTCTGA8950.035.68860616
ATGCCGT9100.034.38006249
TCGTATG9250.034.18728345
GATCGGA9250.033.4153821
GTCACGA10250.031.50096729
ATCTCGT8900.031.228942
ATCGGAA10750.031.0113932
CCGTCTT10550.030.32240152
AGTCACG10600.029.8057728
ACGAGAT10900.028.98999832
TCGGAAG11400.028.9383433
ACGTCTG11700.027.89374415
Produced by FastQC (version 0.11.5)