FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7248853
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87260.12037766526649112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6850.040.07522646
TATGCCG7050.039.4366848
CTCGTAT7400.037.56208444
TCGTATC7400.036.61038238
TCTCGTA7950.034.96127343
GCCGTCT7900.034.7622351
GATCGGA8150.034.0894281
TGCCGTC8100.033.9013250
ATGCCGT8300.033.5004349
TATCTCG8400.032.25601241
ATCGGAA8850.031.7797952
GTCACGA8850.031.38463629
CGTCTTC9850.029.65027653
ATTCGTA9650.029.51317236
TCGTATG9750.028.51082645
ACACGTC9750.028.48641213
GAATTCG9900.028.4141234
ATCTCGT9750.027.4361742
AGTCACG10450.026.91120328
ACGAATT10600.026.86274132
Produced by FastQC (version 0.11.5)