FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6253693
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3200.041.2699348
AGGGGGG24650.033.2676541
CGGCTAT4400.030.7920334
CTCGTAT4050.030.03015144
CGTATGC4500.029.34586146
ATGCCGT4650.029.15030149
ACCGGCT4650.029.13537832
TCACCGG4550.029.01130730
GATCGGA4350.028.756791
TGCCGTC4950.027.38536850
TCTCGTA4700.026.61532643
CACCGGC5300.024.90653231
GTCACCG5300.024.90553729
ATCGGAA5350.024.6765252
ACACGTC5550.024.40994313
CGTCTTC5750.024.18320753
GCCGTCT5350.024.03983351
CGTCTGA6150.021.46330816
TCGGAAG6400.021.1693523
TCGTATG6300.019.85762645
Produced by FastQC (version 0.11.5)