FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7789951
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3150.026.47680348
TGCCGTC5000.018.07076650
AGGGGGG20550.017.4219261
TCTCGTA4400.017.37484643
GATCGGA4450.017.1843641
CTCGTAT4750.016.09459344
CGTATGC5300.015.736011546
GCCGTCT5850.015.44529751
TATCTCG5400.015.44410841
ATCTCGT5200.014.70169642
ATCGGAA6200.014.5743222
ATGCCGT5600.014.27283549
CGTCTGA6050.012.63552116
ACACGTC6500.012.29572913
TCGTATG7200.012.06594145
TCGGAAG6800.011.7538733
CGTCTTC8200.011.01918553
CCGTCTT8350.010.82109552
CGGAAGA8607.2759576E-129.29374
CACGTCT10355.456968E-128.3931614
Produced by FastQC (version 0.11.5)