FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7394103
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC7500.045.8609238
GATCGGA7650.045.4198381
TAATGCG7800.044.98301334
TATGCCG5450.042.72039448
ATGCGCA8600.040.80048436
AATGCGC8550.040.63138235
CGCATCT6700.039.92920339
TGCGCAT8800.039.08468637
ATCGGAA9300.038.103042
TCGGAAG9750.036.342963
CGTCTGA9750.035.98422216
ACACGTC9950.035.6102813
AGGGGGG30850.034.577231
TCTCGTA6250.034.4677443
CGCATCG2550.034.06234439
CTCGTAT6350.033.92585844
TCGCGTA851.1368502E-732.7018443
CGTATGC6900.031.72734646
GCCGTCT6000.029.54284551
CATCGCG1900.029.25835441
Produced by FastQC (version 0.11.5)