FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9952347
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190620.19153271082690346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11300.049.8230748
AGGGGGG89350.047.492121
TCTCGTA12050.045.56406443
CTCGTAT12300.045.20322844
GATCGGA12400.043.7223321
TGCCGTC13400.042.2770950
CGTATGC13550.041.8046646
TTACTCG13750.041.18955635
GCCGTCT13950.039.61507451
ACACGTC14800.039.2036413
ATGCCGT14750.038.8777549
TACTCGA14750.038.16186536
ATCTCGT14300.038.151542
ATCGGAA14750.037.9262962
TCGTATG15600.036.75571445
CGTCTGA15700.036.7342716
TCGGAAG16200.034.52943
CGTCTTC17450.031.67158753
CCGTCTT17700.031.22251952
ACGTCTG19200.031.12362315
Produced by FastQC (version 0.11.5)