FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7920000
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAT5650.036.2847134
CGTATGC5700.034.7535946
TATGCCG5600.034.1355848
GATCGGA5750.033.2419321
CTCGTAT6350.030.64728544
TGCCGTC6550.030.24778450
TCACCGG7000.029.28583530
ATGCCGT6700.029.0505349
TCTCGTA6800.028.10774443
GCCGTCT7100.027.41578351
GTCACCG7500.026.4058929
ACCGGCT7800.025.83702732
ACACGTC7800.025.83637613
ATCTCGT7700.025.72489542
ATCGGAA7750.025.1078322
CACCGGC8200.025.00010531
TCGTATG8000.024.7611545
CGTCTGA8900.022.25215516
AGGGGGG24200.021.684681
CGTCTTC9350.021.56423453
Produced by FastQC (version 0.11.5)