FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6584356
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5650.041.8277348
CGATAGA6200.038.66570336
AGCGATA6750.035.51354634
TCTCGTA6300.034.19577443
CTCGTAT6650.033.96509644
CGTATGC7100.033.77211846
GCCGTCT7250.033.55882651
TGCCGTC7700.032.0477750
GCGATAG8200.029.65784335
ACACGTC8400.029.36239813
ATGCCGT8300.028.89291249
GATCGGA8250.028.2190361
CGTCTGA8450.027.95533216
TCGTATG8700.027.56052445
CAGCGAT8600.027.46961433
ATCTCGT8750.025.81190342
ATCGGAA9050.025.7227762
TCGGAAG9700.024.7154753
CCGTCTT9700.024.36746252
CGTCTTC9700.024.00984853
Produced by FastQC (version 0.11.5)