FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4605703
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC450.003889785431.7697568
CGGCTAT2402.1372944E-715.92284734
TATGCCG2302.3875764E-615.11091148
TCTCGTA2556.968263E-613.6272343
ACCGGCT2801.8303965E-512.40741332
CTCGTAT2852.1887374E-512.19304944
ATCGGAA3459.565865E-712.0845292
CGTATGC3256.4652013E-611.76262546
GCCGTCT3551.3434546E-611.74922451
TGCCGTC3551.3436311E-611.74909650
GATCGGA2752.1244294E-411.3714311
TCGTATG3602.0124962E-510.6184645
ATGCCGT3351.1343525E-410.37488149
TCACCGG3602.3565671E-49.64968630
CGTCTTC4703.643996E-69.61426153
CGTCTGA4452.0097761E-59.3674816
ACACGTC4159.628536E-59.20749313
ATCTCGT3804.0415453E-49.14448842
CACCGGC4108.6421805E-48.47307931
GTCACCG5058.872455E-58.254601529
Produced by FastQC (version 0.11.5)