FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5221234
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAA1155.275797E-827.19002335
TCCGCGA1151.5993483E-624.16821334
CGCGAAA1301.7284947E-724.05317336
GCGAAAT2052.3038899E-413.55842837
TATGCCG2153.440567E-412.93065348
TCTCGTA3103.822246E-612.329864543
GATCGGA2357.27913E-411.8297181
CTCGTAT2650.001987673610.49000244
CTCCGCG2700.002325491310.29377133
ACTCCGC3106.433753E-410.08652532
TCGTATG3501.769341E-49.92832345
CGTATGC3259.905711E-49.62311346
ATGCCGT3000.005544429.26696749
TGCCGTC3400.00149547759.19891650
GCCGTCT3450.00170778519.0658651
ATCTCGT4653.3647237E-58.96700242
CACTCCG4006.756761E-48.68553531
ATCGGAA3900.00518836878.0181082
TCCCGCC6400.0065455147.8130370
CGGAAGA5800.00345603776.5892794
Produced by FastQC (version 0.11.5)