FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7328241
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN187230.2554910516725637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGT9800.017.7025139
ACGGCGA1000.00991371817.43236259
GAGCGGG7000.017.32859
AGCGGGG8450.016.4061310
TTGGCGG5950.015.15575635
TGGGCGG17350.013.79338435
AGAGCGG6450.013.4314758
GCGGGGT8700.013.159636538
TAGGCGG3456.905793E-813.06909535
CGGGGGA6850.012.74006361
GAGAGCG7750.012.5198677
GGCGGGG31750.012.12735337
GGGGGCG23600.011.35939156
CGGGTAA2802.4055391E-411.22035661
GCGGGTA2802.4205097E-411.21285360
GTTGGCG4652.4265319E-811.18657334
TTGGGCG5905.4569682E-1111.16761234
GCGGATA2500.001183502511.16301760
GGGGCGG26350.010.57447957
GGGGGGC32100.010.517838555
Produced by FastQC (version 0.11.5)