FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6225243
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA75020.12050935200441171TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC12000.053.87080450
TATGCCG12200.053.26698748
CGTATGC12500.051.98314746
CTCGTAT12100.051.97186344
TCTCGTA12250.051.0477543
GCCGTCT12800.050.24083351
GATCGGA12550.050.0938341
TATCTCG12450.049.94223841
TCGTATG13050.049.52362445
ATGCCGT13350.048.41969349
ATCGGAA13450.048.2862362
CGTCTGA14300.046.13577716
ACACGTC14350.045.97392313
ATCTCGT13750.045.4774442
TCGGAAG15100.043.6953623
CGTCTTC15450.042.9817253
CGGAAGA16800.039.2738084
CCGTCTT16600.038.74334752
CACGTCT17050.038.28721214
ACGTCTG17350.038.22558615
Produced by FastQC (version 0.11.5)