FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6366274
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCA1404.3291948E-1027.29776836
CGCGCAT1807.930794E-921.23193237
TCTCGCG2452.70089E-715.59835734
GCGCATC2807.538074E-814.89004238
TATGCCG2859.333053E-814.63260748
CGTATGC3501.135164E-611.91465546
CTCGTAT3501.475014E-510.92108144
CGCATCT3904.17666E-610.69062239
CTCGCGC3602.0187617E-510.61563135
GATCGGA4301.1720404E-610.5053971
CGTCTGA5452.2171662E-810.19870516
ACTCTCG4602.787434E-69.81848732
GCCGTCT4159.575572E-59.21228751
TCTCGTA4159.596109E-59.21047743
ATCGGAA5402.183804E-69.0080522
GGGGACG3600.00252315058.68469824
TCGGAAG6101.2279197E-68.5436683
ATGCCGT4502.3084745E-48.49530949
ACACGTC5958.093501E-68.17383413
ACGTCTG6908.265888E-78.05549815
Produced by FastQC (version 0.11.5)