FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5271248
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCACAGGCTGTAGAGAAGGCTGGAAGCGGATCTGTTGGCGGATGCCCT166700.31624389518383506No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58810.11156750735309741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGGCG22150.062.90923736
CGGATCT24100.059.94878429
CGGATGC24350.058.8075341
TTGGCGG23650.058.77340337
AGCGGAT24800.058.53008327
ACGCCTT25150.058.03419552
GCGGATG24800.057.7371640
TGGCGGA24900.057.36024538
AAGCGGA25900.055.91229626
CCTCACG26100.055.6571448
GAAGCGG27400.052.97512425
GGAAGCG28150.051.80697624
CGCCTTG28250.051.6638853
CTCAATT50850.048.02120270
GCGGATC32050.045.6159628
GGCGGAT32500.043.84318539
GATGCCC34600.041.88928243
GGATGCC34850.041.68772542
GCTGTAG36500.040.9907729
GGCTGTA37600.039.975848
Produced by FastQC (version 0.11.5)