FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4821000
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3800.044.8108748
CGGCTAT4250.040.05579834
TCTCGTA4350.039.14106843
CTCGTAT4400.038.69668644
CGTATGC4500.037.06611646
GATCGGA5100.032.708791
TGCCGTC5400.031.53488350
ACACGTC5350.031.1696713
ATGCCGT5600.031.02825749
TCACCGG5450.030.598730
ACCGGCT5350.030.52156832
TCGTATG5700.030.48168845
GTCACCG5700.029.2566529
ATCTCGT6000.028.955542
ATCGGAA5950.028.6151452
CACCGGC6100.027.33818231
GCCGTCT6350.026.81873751
TCGGAAG6300.026.4725023
CGTCTTC6400.026.06779353
CGTCTGA6450.025.85444816
Produced by FastQC (version 0.11.5)