FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7582931
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA2600.050.78252834
TCGCGTA503.6592355E-641.7003543
AGGGGGG34300.041.4432261
CGCGAAA3300.040.01179536
CCGCGAA3400.039.85642235
CTCCGCG4500.030.11294733
CGCGTAT703.6678495E-529.78655444
GATCGGA4650.028.4024011
GCGAAAT5550.025.04327237
ACTCCGC6200.022.97700132
CGTCTGA7300.020.94234316
TATGCCG3750.020.38885748
CACTCCG7500.019.9206131
ACACGTC7200.019.30329713
ATCGGAA6900.019.1383482
AATCGCG1501.5940508E-518.53287941
TCGGAAG8250.017.2697283
ATCGCGT1250.001850421716.6800342
CGGAAGA9650.014.7641784
ACGTCTG10050.014.1746815
Produced by FastQC (version 0.11.5)