FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5215932
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN123880.23750309628269695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7550.047.40476248
CGTATGC8000.044.26454546
CTCGTAT8050.041.8001644
GCCGTCT8700.041.2059151
GATCGGA8750.040.303511
TCGTATG9150.039.06708545
TCTCGTA8550.038.9353343
ACACGTC9550.038.72368613
TGCCGTC9350.038.3250150
ATCGGAA9700.038.129632
TACTCGA9300.037.21150636
TTACTCG9350.037.0053935
ATGCCGT9600.036.9409749
ATCTCGT9150.036.750942
TCGGAAG10200.035.9219933
CGTCTGA10150.035.75392516
CGTCTTC10250.035.02290753
CGGAAGA10800.033.924044
CCGTCTT10400.033.824952
ACGTCTG11550.031.42011615
Produced by FastQC (version 0.11.5)