FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5215932
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN149160.2859699858050297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATA1600.00949590613.04097150
AGGGGGG22700.05.67062951
TAGGGGG8000.00257919925.651087350
GGGGGCG13503.0414885E-65.43189656
GGGGGAT13805.3987405E-65.263994734
Produced by FastQC (version 0.11.5)