FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7201504
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT4150.051.89742737
TCGCGCA4350.049.5106536
TCTCGCG5150.041.8173534
TATGCCG5950.036.79628448
GCGCATC6100.034.73972738
CTCGCGC6400.034.1933535
CGTATGC7100.031.81215546
GATCGGA7050.031.0433621
ATGCCGT7200.029.9255849
CTCGTAT7200.029.43758644
ACTCTCG7400.029.09958332
GCCGTCT7600.028.35409751
TCTCGTA7500.028.25890543
TGCCGTC7800.028.07051850
ATCGGAA8400.027.7044452
TCGTATG8200.027.12033845
CGCATCT8000.026.92347539
ACACGTC8650.026.4994313
ATCTCGT9050.023.80157742
TCGGAAG9400.023.6481423
Produced by FastQC (version 0.11.5)