FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5212978
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA1800.048.25191534
CGCGAAA2050.042.36956436
CCGCGAA2300.037.76309235
TATGCCG2600.033.4202548
CTCCGCG2850.030.47401433
GATCGGA3400.025.550281
GCGAAAT3700.025.35400837
CGTATGC3650.023.80414846
CTCGTAT3700.023.48111544
TCTCGTA3900.022.27674343
GCCGTCT4000.021.7248351
ATGCCGT3950.021.11902249
TCGTATG4200.020.6865445
CACTCCG4450.019.51650431
TGCCGTC4400.018.9596750
ACTCCGC4850.018.62370932
ATCGGAA4850.017.1930962
ATCTCGT5300.017.04752242
ACACGTC5400.016.72540313
CGTCTTC5500.015.16890153
Produced by FastQC (version 0.11.5)