FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7651867
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA138240.1806617914294642TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC19150.058.5799338
TATGCCG20000.057.8605148
TAATGCG20450.056.3749234
AATGCGC21050.055.26437835
GATCGGA20250.055.2190741
CGTATGC20900.055.02844246
ATGCGCA21250.054.41883536
CTCGTAT20850.053.8198244
TGCGCAT21650.052.77456337
GCCGTCT21800.052.6175651
ATGCCGT21800.052.44952849
CGCATCT21300.052.3428739
TCGTATG22150.052.2322945
TGCCGTC22200.051.6647650
TCTCGTA21700.051.54921343
CGTCTGA22750.050.9707516
ATCGGAA22950.050.834772
TCGGAAG23400.050.1536183
ACACGTC23300.050.0655913
CATCTCG22950.048.58474741
Produced by FastQC (version 0.11.5)