FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7645756
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA82730.10820381921683088TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14000.053.6222148
CGTATGC14650.052.18996446
GCGATAG14900.050.1388835
AGCGATA15000.050.03495834
TCTCGTA14550.048.7234343
GATCGGA15300.048.606991
CTCGTAT14800.048.13646744
CGATAGA15400.047.83503336
GCCGTCT16400.046.2043551
TGCCGTC16350.046.13123350
CGTCTGA17200.045.04570416
ATGCCGT16900.044.42280249
ATCTCGT15750.044.12744542
TCGTATG17250.043.31544545
ATCGGAA17850.042.439942
ACACGTC17950.042.38933613
ACAGCGA18150.041.92360732
CGTCTTC17850.041.67431653
CCGTCTT18200.041.4445152
CAGCGAT18150.041.15895533
Produced by FastQC (version 0.11.5)