FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7645756
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGCG24950.08.77280956
TAGGGGG14550.08.59420750
GGGGGGC32750.07.31973155
GGGGGGA41000.07.28133252
GGGGCGG28950.07.201069457
GGGGGGT44150.07.00274414
CCCTCCG8200.0063906596.909960370
AGAGCGG8955.6561257E-66.5957528
GGGCGGG29850.06.518738758
AGGGGGG35200.06.51309651
GAGAGCG8106.7569636E-56.4302887
GGGGAGA41950.06.28948164
GGGGGAG44600.06.07117943
GGGGGTT27800.05.998417415
CGGGGGA8306.3218956E-45.86235661
GAGCGGG9053.079546E-45.75634059
AGCGGGG11253.1354524E-55.557114610
GGGTTGG31700.05.4799517
CGGGGGG23407.2759576E-125.34400912
GGGGTTG31400.05.200231616
Produced by FastQC (version 0.11.5)