FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4758618
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT2800.032.25839237
TCTCGTA3050.029.62111343
CTCGTAT3150.028.68106344
TCTCGCG3150.028.67141534
TATGCCG3300.027.3811248
GATCGGA3200.026.0554031
TCGCGCA3650.025.69740136
CGTATGC3650.024.75449246
TGCCGTC4000.024.33039950
GCCGTCT3900.024.0650651
TCGTATG4100.022.8836945
CGTCTTC4500.021.63521653
ACTCTCG4400.021.31559232
ACACGTC4400.021.31245413
CTCGCGC4350.020.76358635
ATCGGAA4700.020.6949672
CGTCTGA4400.020.52353716
ATCTCGT4750.019.75015842
ATGCCGT4950.019.65865349
TCGGAAG4950.018.9463963
Produced by FastQC (version 0.11.5)