FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6914992
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN73650.10650771541022752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9100.049.9843831
TATGCCG10050.047.02952248
TCTCGTA9700.045.4808243
TGCCGTC10850.044.21277650
GCCGTCT10800.044.1000851
CTCGTAT10150.043.80857544
CGTATGC10900.043.0360746
ATGCCGT11200.042.20610449
ATCGGAA11450.041.235852
TCGTATG11900.039.7069945
ATCTCGT11150.039.56340842
TCGGAAG12550.037.3443833
CGTCTGA13000.036.3111216
ACACGTC13200.035.49813
CCGTCTT13800.034.2649152
CGGAAGA13900.033.7144784
CGTCTTC14100.033.53951653
ACGTCTG14750.032.4736515
CACGTCT15450.030.55304514
AGGGGGG29350.030.2856081
Produced by FastQC (version 0.11.5)