FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5462067
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA506.575465E-848.6426243
AATCGCG1550.038.1043341
GATCGGA6150.037.2832681
ACACGTC6250.037.23236513
CCGGAGA6650.034.4743335
CTCCGGA7100.032.7773733
CGCGTAT851.1368684E-732.7015244
CGGAGAA7200.032.32390636
ACTCCGG7150.032.0614832
CGTCTGA7350.031.66017516
CACTCCG7450.030.77013431
TATGCCG2850.030.48397648
TCCGGAG7550.030.36369734
ATCGCGT1501.8189894E-1230.11099442
ATCGGAA7700.029.773832
TCTCGTA3850.028.87873543
TCGGAAG8150.028.5560873
CTCGTAT3700.028.17191744
ACGTCTG8550.027.2166415
CGGAAGA8650.026.903974
Produced by FastQC (version 0.11.5)