FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7744113
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAT3550.017.61306434
TATGCCG4301.8189894E-1214.54797448
CGTATGC4850.014.32994746
ATGCCGT4951.8189894E-1213.34058849
TGCCGTC5205.456968E-1212.69986950
CTCGTAT5255.456968E-1212.5752644
GCCGTCT5651.8189894E-1212.30395151
TCTCGTA4903.274181E-1012.05484143
ACCGGCT5652.3646862E-1111.68074832
TCACCGG5753.274181E-1111.47745730
GATCGGA5251.033186E-911.2507491
CACCGGC6502.7284841E-1110.68764931
TCGTATG6959.094947E-1210.49963145
GTCACCG6402.382876E-1010.31171129
CGTCTGA6604.1654857E-109.99904116
ATCTCGT7252.186971E-88.62627942
ACACGTC8001.3322278E-88.24920813
CCGTCTT8453.9963197E-98.22727552
ATCGGAA8051.2896271E-77.76755332
ACGTCTG9101.6094418E-87.63373915
Produced by FastQC (version 0.11.5)