FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6444933
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT6700.039.41614544
TATGCCG6800.039.35758648
GATCGGA6800.038.824141
CGTATGC7400.037.1012146
TCTCGTA7300.036.6513343
TCGTATC7150.036.4423438
TATCTCG7700.036.09779441
TCGTATG7950.034.5315145
TGCCGTC7850.034.09557750
GTCACGA8050.033.22216429
ATGCCGT8250.032.8622649
GCCGTCT8400.031.86436851
ATTCGTA8450.031.65467836
ATCTCGT8500.031.47604842
CGTCTGA8700.031.13830416
ATCGGAA9050.029.9375462
ACACGTC9050.029.54960313
CGAATTC9350.029.34802633
AATTCGT9350.028.6061535
AGTCACG9750.028.14203828
Produced by FastQC (version 0.11.5)