FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5829075
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA186470.31989638150135313TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA26000.061.4661641
TATGCCG26600.061.3979248
CGTATGC26650.061.27851546
TTACTCG27250.060.16568835
TGCCGTC27050.059.99678850
TCTCGTA24100.059.9727643
GCCGTCT27100.059.8891851
CTCGTAT25450.059.795444
TACTCGA26900.059.3994836
TCGTATG27800.058.74159645
ATCGGAA28700.057.977142
ATGCCGT28100.057.87510749
ACACGTC28800.057.4026513
CGTCTGA28800.057.4026516
TCGGAAG29200.056.864443
CGTCTTC28800.056.60415353
ATCTCGT25350.056.46632842
CCGTCTT29400.055.56528552
CGGAAGA30750.054.5634044
ACTCGAT29000.053.42261537