FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5829075
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG700.00127481624.83709762
CGGGGGA6250.016.13209761
GAGCGGG6800.015.8282179
TAGGGGG13450.015.49125250
GGGGGCG20750.014.90281756
AGCGGGG7950.014.41230510
GGGGCGG22500.013.59068757
GGGGGGC27350.012.70264955
AGAGCGG6750.011.8282688
GGGGGGT51850.011.78789814
GGGCGGG23300.011.7833458
GAGAGCG6500.011.7475357
GCGGGTA2408.659231E-411.58745660
CGGGGGG20200.011.34570412
GCGGGGG19350.010.94662611
GGGGAGA35100.010.7758714
GGGGGAT21600.010.61690234
CGGGTGA4301.1638422E-610.51108961
GGGGGGA49850.010.2316142
GGGGGAG41800.010.1271383
Produced by FastQC (version 0.11.5)